Utilization of target-specific, hypersensitive strains of Saccharomyces cerevisiae to determine the mode of action of antifungal compounds.
نویسندگان
چکیده
Target-specific hypersusceptible strains of Saccharomyces cerevisiae were used to screen antifungal compounds. Two novel Erg7p inhibitors were identified, providing proof of principle of the approach taken. However, observed hypersensitivities to antifungals acting via other targets imply that use of this tool to identify the mode of action requires significant deconvolution.
منابع مشابه
Isolation of indigenous Glutathione producing Saccharomyces cerevisiae strains
Background: Glutathione (GSH) is a non-protein thiol compound, which plays an important role in the response to oxidative stress and nutritional stress. The aim of this study was to isolate indigenous S. cerevisiae strains capable of effectively produce GSH. Methods: One hundred-twenty sweet frui...
متن کاملCharacterization of an Interesting Novel Mutant Strain of Commercial Saccharomyces cerevisiae
The yeast strains that are resistant to high concentration of ethanol have biotechnological benefits and aresuitable models for physiology and molecular genetics research fields. A novel ethanol-tolerant mutant strain,mut1, derived from the commercial Saccharomyces cerevisiae showed higher ethanol production, and alsodemonstrated resistance to ethanol but not to other alcohols...
متن کاملGrowth and feed utilization of goldfish (Carassius auratus) fed graded levels of brewers yeast (Saccharomyces cerevisiae)
In this study, a feeding trial was conducted to examine the potential of replacing fish meal with brewers yeast in practical diet of goldfish (Carassius auratus). Five isoproteic (37% CP) and isocaloric (3350 kcal/kg) diets were formulated to contain graded levels of brewers yeast. Fish meal protein was replaced by 0%, 15%, 25%, 35%, and 45% of yeast. Each diet was randomly allocated to triplic...
متن کاملGreen synthesis of silver nanoparticles: Another honor for the yeast model Saccharomyces cerevisiae
Background and Purpose: Microorganism-based synthesis of nanostructures has recently been noted as a green method for the sustainable development of nanotechnology. Nowadays, there have been numerous studies on the emerging resistant pathogenic bacteria and fungal isolates, the probable inability of bacteria and fungi to develop resistance against silver nanoparticles’ (SNPs) antibacte...
متن کاملCharacteristics of Saccharomyces cerevisiae isolated from fruits and humus: Their suitability for bread making
The objectives of this study were to clarify whether the wild yeast isolated from fruits and humus is suitable forbread making. Using colony PCR, assimilation of carbohydrate and 18S rRNA sequencing, seven strains fromamong 70 samples were identified as Saccharomyces cerevisiae. The ethanol and CO2 production by the 10-2 wild yeast strain were highest among the strains. The pH and utilized gluc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 49 6 شماره
صفحات -
تاریخ انتشار 2005